Reexamining Michaelis-Menten enzyme kinetics for xanthine oxidase

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Reexamining Michaelis-Menten enzyme kinetics for xanthine oxidase.

Abbreviated expressions for enzyme kinetic expressions, such as the Michaelis-Menten (M-M) equations, are based on the premise that enzyme concentrations are low compared with those of the substrate and product. When one does progress experiments, where the solute is consumed during conversion to form a series of products, the idealized conditions are violated. Here, we analyzed data of xanthin...

متن کامل

A Personal View Reexamining Michaelis-Menten enzyme kinetics for xanthine oxidase

Bassingthwaighte JB, Chinn TM. Reexamining Michaelis-Menten enzyme kinetics for xanthine oxidase. Adv Physiol Educ 37: 37–48, 2013; doi:10.1152/advan.00107.2012.—Abbreviated expressions for enzyme kinetic expressions, such as the Michaelis-Menten (M-M) equations, are based on the premise that enzyme concentrations are low compared with those of the substrate and product. When one does progress ...

متن کامل

Conformational Nonequilibrium Enzyme Kinetics: Generalized Michaelis-Menten Equation.

In a conformational nonequilibrium steady state (cNESS), enzyme turnover is modulated by the underlying conformational dynamics. On the basis of a discrete kinetic network model, we use an integrated probability flux balance method to derive the cNESS turnover rate for a conformation-modulated enzymatic reaction. The traditional Michaelis-Menten (MM) rate equation is extended to a generalized f...

متن کامل

Parallel versus Off-Pathway Michaelis-Menten Mechanism for Single-Enzyme Kinetics of a Fluctuating Enzyme.

Recent fluorescence spectroscopy measurements of the turnover time distribution of single-enzyme turnover kinetics of β-galactosidase provide evidence of Michaelis-Menten kinetics at low substrate concentration. However, at high substrate concentrations, the dimensionless variance of the turnover time distribution shows systematic deviations from the Michaelis-Menten prediction. This difference...

متن کامل

One hundred years of Michaelis–Menten kinetics

0.1016/j.pisc.2014 he Author. Publish mons.org/licenses t of an special issu etics”. Copyright thor. [email protected] Abstract The year 2013 marked the centenary of the paper of Leonor Michaelis and Maud Menten (Michaelis and Menten, 1913), and the 110th anniversary of the doctoral thesis of Victor Henri (Henri, 1903). These publications have had an enormous influence on the progress of biochem...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Advances in Physiology Education

سال: 2013

ISSN: 1043-4046,1522-1229

DOI: 10.1152/advan.00107.2012